The effect of different gelling agents as part of culture medium on the rate of cucumber (<i>Cucumis sativus</i> L.) unpollinated ovules gynogenic development

Relevance. The induction of gynogenic development the culture unpollinated ovules Cucumis sativus L., as a part solid nutrient media agar-agar or Phytagel™ are used gelling agent. agent determines mobilization substances in composition medium and has various effects on explants, which affects quality resulting regenerates. However, there no scientific works that explaines effect these agents compare them with each other for C. L. aim: investigate gynogenesis cucumber ovules. Materials methods. There two promising collection specimens №58 №831 laboratory cucurbits crop breeding seed production FSBSI FSVC were included into research. IMC (Induction Medium Cucurbitaceae) 30 g/l sucrose, 200 mg/l ampicillin, 0.2 thidiazuron (TDZ) was gynogenesis; at concentration 7 3.5 gel-forming agents. Ovules isolated from ovaries phase half-opened flower (FL-1) fully opened (FL). Cultivation carried out plastic Petri dishes diameter 60 mm an air gap 28.8 cm 3 – "KS No. 1", glass jars 140 2". Results. On containing medium, area during days cultivation increases irregularly. Coefficients ovule enlargement between differed 1.7 to 2.6 times depending time. average growth rate 0.08 2 /day, while it 0.02 /day. Gelling type phenotype significant factors affecting increase entered culture. Herewith share 55.01%, genotype 14.53%. stage vessel not found both genotypes. In study, possible achieve 67% agar-agar. Conclusion. faster using than agaragar. At same time, percentage induced significally higher phenotypes.